GN Broth IP (Medium 11)

203070110010 / 203070110100

GN Broth is used for isolation of Shigella species in compliance with IP.

GN Broth is recommended by the Indian Pharmacopoeia for the selective isolation of Shigella species with subsequent isolation on a selective medium, XLD Agar. Croft and Miller isolated more strains of Shigella from rectal swabs using this medium. Taylor and Schelhart showed the superiority of GN Broth to selenite enrichment media for isolation of Shigella.

Composition*

Ingredients
g/L
Polypeptone Peptone
20.0
Glucose
1.0
Sodium Citrate
2.0
Sodium Deoxycholate
0.5
Di-Potassium Hydrogen Phosphate
4.0
Mono-Potassium Dihydrogen Phosphate
1.5
Sodium Chloride
5.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 7.0 ± 0.2

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