Reinforced Medium for Clostridia is used for cultivation and enumeration of Clostridia from pharmaceutical products in accordance with microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.
Reinforced Medium for Clostridia can be used to initiate growth from small inocula and to obtain the highest viable count of Clostridia. It can be used in studies of spore forming anaerobes, especially Clostridium butyricum in cheese, for enumeration of Clostridia in tube dilution counts or for preparation of plates for isolation. Other spore forming anaerobes, Streptococci and Lactobacilli also grow in this media. This is an enriched but non-selective medium.
Composition*
| Ingredients | g/L |
| Beef Extract | 10.0 |
| Peptone | 10.0 |
| Yeast Extract | 3.0 |
| Soluble Starch | 1.0 |
| Glucose Monohydrate | 5.0 |
| Cysteine Hydrochloride | 0.5 |
| Sodium Chloride | 5.0 |
| Sodium Acetate | 3.0 |
| Agar | 0.5 |
*Adjusted to suit performance parameters.
Final pH (at 25°C): 6.8 ± 0.2
