Hugh Leifson Medium BIS

201080180500

MSDS

Technical Data

APPLICATION

Clinical Microbiology

Veterinary

Water

Food

Dairy

Hugh Leifson Medium BIS is a medium used to distinguish between anaerobic and aerobic breakdown of carbohydrate (glucose) as per BIS specification IS:5887 (Part VII)-1999.

Composition*

Ingredients	g/L
Peptone	2.0
Sodium chloride	5.0
Dipotassium phosphate	0.3
Glucose (Dextrose)	10.0
Bromothymol blue	0.03
Agar	3.0

*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.1 ± 0.2

g/L: Suspend 20.33g of the powder in 1000 mL purified / distilled water.

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Products+

0.1% Peptone Water
0.1% Peptone Water I
0.1% Peptone Water II
0.1% Peptone Water IV
0.1% Peptone Water with 0.1% Polysorbate 80
0.1% Peptone Water with 1% Polysorbate 80
0.1% Peptone Water with 1% Polysorbate 80 II
Acetobacter Broth (Mannitol)
Alicyclobacillus Agar
Alternative Thioglycollate Medium
Alternative Thioglycollate Medium
(Sterility Testing Medium)
Alternative Thioglycollate Medium
(Sterility Testing Medium) I
Antibiotic Assay Medium No.4
(Yeast Beef Agar)
Arginine-Glucose Yeast Extract Agar
Aspergillus Differentiation Medium Base
Azide Dextrose Broth
Bacillus Cereus Agar Base
Baird Parker Agar Base
Baird Parker Agar Base (Agar Medium O) EP
Baird Parker Agar Base
(Agar Medium O) BP
Baird Parker Agar Base BIS
Baird Parker Agar Medium USP
Bile Esculin Agar
Bile Esculin Azide Agar
Bile Salt Agar
Bile Salt Agar BIS
Bismuth Sulphite Agar
Bismuth Sulphite Agar Medium USP
Blood Agar Base No. 2
Brilliant Green Agar, Medium USP
Brilliant Green Agar, Modified
Brilliant Green Bile Broth 2%
Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Agar Medium L) BP
Brilliant Green, Phenol Red, Lactose Monohydrate, Sucrose Agar (Agar Medium L) EP
Buffered Peptone Water
Buffered Peptone Water BIS
Buffered Peptone Water ISO
Buffered Peptone Water with NaCl
Buffered Sodium Chloride-Peptone Solution pH 7.0 (Harmonized)
Buffered Sodium Chloride-Peptone Solution pH 7.0 (Harmonized)
Buffered Sodium Chloride-Peptone Solution pH 7.0 (Harmonized) I
Buffered Sodium Chloride-Peptone Solution pH 7.0 (Harmonized) III
Buffered Sodium Chloride-Peptone Solution pH 7.0 with 0.05% Polysorbate 80
Buffered Sodium Chloride-Peptone Solution pH 7.0 with 0.1% Polysorbate 80
Buffered Sodium Chloride-Peptone Solution pH 7.0 with 0.1% Polysorbate 80 and 0.7% Soya Lecithin
Buffered Sodium Chloride-Peptone Solution pH 7.0 with 1% Polysorbate 80 and 0.5% Soya Lecithin
Burkhloderia Cepacia Selective Agar Base USP
Burkholderia Cepacia Agar Base
Burkholderia Cepacia Selective Agar USP Plate (Triple Layer Pack, Gamma-Irradiated)
Campylobacter Agar Base
Carbohydrate Consumption Broth Base
Cetrimide Agar (Harmonized)
Cetrimide Agar (Harmonized)
Cetrimide Agar Base
Cetrimide Agar Plate (Harmonized)
Chloramphenicol Yeast Glucose Agar
Clostridial Agar
Clostridium Butyricum Agar
Columbia Agar (Harmonized)
Columbia Agar (Harmonized)
Columbia Agar Plate (Harmonized)
Columbia Blood Agar Base E
Cooked Meat Medium
(Robertson Cooked Meat Medium)
Corn Meal Agar
Czapek Dox Agar
Czapek Dox Broth
Decarboxylase Agar Base
Decarboxylase Broth Base, Moeller
(Moeller Decarboxylase Broth Base)
Deoxycholate Citrate Agar
Deoxycholate Citrate Agar (Agar Medium J) EP
Deoxycholate Citrate Agar BIS
Deoxycholate Citrate Agar BP
Deoxycholate Lactose Agar
Dey Engley Neutralizing Broth
Dey-Engley Neutralizing Agar
(D/E Agar Disinfectant Testing)
Dey-Engley Neutralizing Broth I
Dey-Engley Neutralizing Broth
(D/E Broth Disinfectant Testing)
Dichloran Glycerol Medium Base ISO
Dichloran Rose Bengal Chloramphenicol (DRBC) Agar
Differential Reinforced Clostridial Agar Base
Differential Reinforced Clostridial Broth
Diluting Fluid A
Diluting Fluid A
Diluting Fluid A I
Diluting Fluid D
Diluting Fluid D
Diluting Fluid D I
Diluting Fluid K
Diluting Fluid K
DNase Test Agar Base
E. coli Identification Kit
E.C. Broth
Egg Yolk Agar Base
Elliker Broth
Ellners Broth
EMB Agar, Levine
EMB Agar, Levine BIS
EMB Agar, Levine Plate
EMB Agar, Levine USP
EMswab^TM - Buffered Sodium Chloride Peptone Solution pH 7.0 with Polysorbate 80 I
EMswab^TM - Buffered Sodium Chloride Peptone Solution pH 7.0 with Polysorbate 80 II
EMswab^TM - Saline with 0.07% Soya Lecithin and 0.5% Polysorbate 80 I
EMswab^TM - Twin Pack System (Swab in Saline + Soyabean Casein Digest Medium with Lecithin and Polysorbate-80) II
EMswab^TM – 0.1% Peptone Water II
EMswab^TM – Twin Pack System (Saline with Swab Stick + Soyabean Casein Digest Medium) I
EMswab^TM – Twin Pack System (Saline with Swab Stick + Soyabean Casein Digest Medium) II
EMswab^TM – Twin Pack System (Swab in Saline with Lecithin and Polysorbate 80 + Soyabean Casein Digest Medium) II
EMswab™ - Saline I
EMswab™- Saline II
EMswab™- Buffered Sodium Chloride Peptone Solution pH 7.0 I
EMswab™- Buffered Sodium Chloride Peptone Solution pH 7.0 II
EMswab™- Buffered Sodium Chloride Peptone Solution pH 7.0 with Lecithin and Polysorbate 80 II
EMswab™- Dey Engley Neutralizing Broth I
EMswab™- Dey Engley Neutralizing Broth II
EMswab™- Saline with Lecithin and Polysorbate 80 II
EMswab™- Soyabean Casein Digest Medium I
EMswab™- Soyabean Casein Digest Medium II
EMswab™- Soyabean Casein Digest Medium with Lecithin and Polysorbate 80 II
Endo Agar
Enterobacteria Enrichment Broth, Mossel (Harmonized)
Enterobacteria Enrichment Broth, Mossel (Harmonized)
Enterobacteria Enrichment Broth, Mossel
(Medium 5) IP
Enterobacteriaceae Identification Test Kit
Fluid Casein Digest Soya Lecithin
Polysorbate 20 Medium (Twin Pack) USP
Fluid Lactose Medium
Fluid Lactose Medium
Fluid Lactose Medium USP
Fluid Selenite Cystine Medium (Twin Pack)
Fluid Selenite Cystine Medium (Twin Pack) ISO
Fluid Selenite Cystine Medium (Twin Pack) USP
Fluid Tetrathionate Medium USP
Fluid Thioglycollate Medium
Fluid Thioglycollate Medium BP
Fluid Thioglycollate Medium EP
Fluid Thioglycollate Medium IP
Fluid Thioglycollate Medium USP
Fluid Thioglycollate Medium VEG
Fluid Thioglycollate Medium
(Sterility Testing Medium)
Fluid Thioglycollate Medium
(Sterility Testing Medium) I
Fluid Thioglycollate Medium
(Sterility Testing Medium) II
Fluid Thioglycollate Medium
(Sterility Testing Medium) III
Fraser Broth Base
Fraser Broth Base ISO
Fraser Secondary Enrichment Broth Base
Gelatin Agar
Glucose Agar
Glucose Broth
Glucose Broth
Glucose Salt Teepol Broth (Twin Pack)
Glucose Yeast Extract Agar
GN Broth IP (Medium 11)
GN Broth IP (Medium 11)
Hektoen Enteric Agar
Hugh Leifson Medium
Indole Nitrate Medium
(Tryptone Nitrate Medium)
Iron Sulphite Agar
Iron Sulphite Agar, Modified
K Agar
KF Streptococcus Agar Base
KF Streptococcus Agar Base w/BCP
King's Medium A Base
King's Medium B Base
King's Medium B Broth
Kligler Iron Agar
Kligler Iron Agar ISO
Lactobacilli MRS Broth W/O Glucose
Lactobacillus Bulgaricus Agar Base
Lactobacillus Identification Kit
Lactobacillus MRS Agar
Lactobacillus MRS Broth
Lactose Broth
Lactose Broth BIS
Lactose Monohydrate Broth
(Broth Medium D) EP
Lactose Monohydrate Broth
(Broth Medium D) BP
Lauryl Tryptose Broth
(Lauryl Sulphate Broth)
Listeria Enrichment Broth
Listeria Identification Agar Base (PALCAM)
Listeria Identification Agar Base (PALCAM) VEG
Listeria Identification Broth Base (PALCAM)
Listeria Identification Kit
Listeria Oxford Medium Base
Listeria Selective Agar
Lysine Decarboxylase Broth
Lysine Iron Agar
M 17 Agar
M-CP Agar
M-Endo Agar LES
M-Endo Agar LES plate
M-Endo Broth
M-MacConkey Broth
M17 Broth
MacConkey Agar (Harmonized)
MacConkey Agar Base
MacConkey Agar BIS
MacConkey Agar Plate (Harmonized)
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts (Medium 8) IP
MacConkey Agar with Crystal Violet,
NaCl and 0.15% Bile Salts
MacConkey Agar without Crystal Violet and with 0.15% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.15% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.5 % Sodium Taurocholate
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Bile Salts
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Taurocholate
MacConkey Broth (Harmonized)
MacConkey Broth (Harmonized)
MacConkey Broth (Harmonized) III
MacConkey Broth Double Strength with Neutral Red BIS
MacConkey Broth Double Strength
with Neutral Red IP
MacConkey Broth Purple with BCP
MacConkey Broth Purple with BCP (Medium 7) IP
MacConkey Broth with Neutral Red
MacConkey Broth with Neutral Red BIS
MacConkey Sorbitol Agar Base
Malt Extract Yeast Extract Glucose Agar
(MY 40G Agar)
Mannitol Motility Test Medium
Mannitol Salt Agar
Mannitol Salt Agar (Harmonized)
Mannitol Salt Agar Plate (Harmonized)
Maximum Recovery Diluent
Michrom^TM Chromogenic Coliform Agar ISO
Michrom^TM Chromogenic Coliform Agar with SLS
Michrom^TM Coliform Agar (Chromogenic Coliform Agar)
Michrom^TM E. Coli Agar
Michrom^TM E. Coli Agar Plate
Michrom^TM Hydrocheck
Michrom^TM Vibrio Agar
MIU Medium Base
Modified Listeria Oxford Agar Base
Modified Oxford Agar
Modified Rappaport Vassiliadis Medium
Moeller Decarboxylase Broth with Arginine
Moeller Decarboxylase Broth with Lysine
Moeller Decarboxylase Broth with Ornithine
Motility Test Medium
Motility Test Medium (Edwards and Ewing) BIS
MR -VP Medium (Glucose Phosphate Broth or Buffered Glucose Broth) BIS
MR -VP Medium
(Glucose Phosphate Broth or
Buffered Glucose Broth)
MRS Broth (De Man, Rogosa and Sharpe Broth)
MRS Broth, Modified (Lactobacillus Heteroferm screen Broth)
Mueller Kauffmann Tetrathionate Broth Base (Novobiocin Broth)
MUG EC Broth
MYP Agar
(Phenol Red Egg Yolk Polymyxin Agar Base)
Nutrient Agar
Nutrient Agar BIS
Nutrient Agar No. 2
Nutrient Agar VEG
Nutrient Agar with 1% Peptone
Nutrient Broth
Nutrient Broth VEG
Nutrient Broth with 1% Peptone
O F Basal Medium
OFPBL Agar Base (Oxidation Fermentation Polymyxin Bacitracin Lactose Agar Base)
Orange Serum Agar
Peptone Salt Broth
Peptone Water
Peptone Water
Peptone Water BIS
Perfringens Agar Base
Pfizer Selective Enterococcus Agar
Phenol Red Agar Base
Phenol Red Broth Base
Phenol Red Dextrose Agar
Phenol Red Dextrose Broth
Phenol Red Lactose Agar
Phenol Red Lactose Broth
Phenol Red Maltose Broth
Phenol Red Mannitol Broth
Phenol Red Sorbitol Broth
Phenol Red Sucrose Agar
Phenol Red Sucrose Broth
Phenylalanine Agar
Phosphate Buffer USP
Phosphate Buffered Saline, pH 7.2
Pikovskaya’s Agar
Pikovskaya’s Broth
Plate Count Agar (Standard Methods Agar)
Plate Count Agar
(Standard Methods Agar) BIS
Plate Count Agar Plate (Gamma-Irradiated)
PNY Medium
Potato Dextrose Agar
Potato Dextrose Agar (Harmonized)
Potato Dextrose Agar for Trichoderma
Potato Dextrose Broth
Potato Dextrose Broth for Trichoderma
Potato Infusion Agar
Pseudomonas Agar Base
Pseudomonas Agar for Detection of Fluorescein Plate
Pseudomonas Agar for Detection of Pyocyanin Plate
Pseudomonas Agar Medium for Detection of Fluorescein
Pseudomonas Agar Medium for Detection of Pyocyanin
Pseudomonas Isolation Agar Base
Purple Agar Base
Rapid Chromogenic Coliform Broth
Rapid Test Kit for Phenylalanine Deamination
Rapid Test Kit for Tryptophan Deamination
Rappaport Vassiliadis Salmonella Enrichment Broth (Harmonized) (Sterile 10 mL Vials)
Rappaport Vassiliadis Salmonella Enrichment Broth IP (Medium 9)
Rappaport Vassiliadis Salmonella Enrichment Broth
(Harmonized)
Reinforced Clostridial Agar
Reinforced Clostridial Agar Plate
Reinforced Medium for Clostridia (Harmonized)
Reinforced Medium for Clostridia (Harmonized)
Reinforced Medium for Clostridia (Medium 15) IP
Rhizobium Medium
Rogosa S L Agar
Rose Bengal Agar Base
Rose Bengal Chloramphenicol Agar
Rose Bengal Chloramphenicol Agar Plate (Gamma-Irradiated)
Sabouraud Chloramphenicol Agar
Sabouraud Chloramphenicol Agar
Sabouraud Chloramphenicol Agar Plate (Triple Layer Pack, Gamma-Irradiated)
Sabouraud Chloramphenicol Agar Plate
(Triple Layer Pack, Gamma-Irradiated)
Sabouraud Chloramphenicol Agar Slant
Sabouraud Dextrose Agar
Sabouraud Dextrose Agar
Sabouraud Dextrose Agar (Harmonized)
Sabouraud Dextrose Agar (Harmonized)
Sabouraud Dextrose Agar Plate
(Triple Layer Pack, Gamma-Irradiated)
Sabouraud Dextrose Agar Plate
(Triple Layer Pack, Gamma-Irradiated)
Sabouraud Dextrose Agar Slant
Sabouraud Dextrose Agar with 1% Glycerol Plate (Triple Layer Pack, Gamma-Irradiated)
Sabouraud Dextrose Agar with Antibiotic(S)
(Medium 4) IP
Sabouraud Dextrose Agar, Modified
(Dextrose Agar Base, Emmons)
Sabouraud Dextrose Agar
with Soya Lecithin and Polysorbate 80
Sabouraud Dextrose Broth (Harmonized)
Sabouraud Dextrose Broth (Harmonized)
Sabouraud Dextrose Broth (Harmonized) III
Sabouraud Glucose Agar
Sabouraud Glucose Broth
Saline
Saline I
Salmonella Selective Enrichment Broth
Salt Meat Broth
Selenite Cystine Broth Base without Biselenite
Selenite F Broth
Selenite F Broth (Twin Pack)
Shigella Broth Base
Simmons Citrate Agar
Simmons Citrate Agar BIS
Simmons Citrate Agar Slant
Skim Milk Agar
Soyabean Casein Digest Agar (Harmonized)
Soyabean Casein Digest Agar (Harmonized)
Soyabean Casein Digest Agar (Harmonized) Slant
Soyabean Casein Digest Agar (Tryptone Soya Agar)
Soyabean Casein Digest Agar
(Casein Soyabean Digest Agar) IP (Medium 2)
Soyabean Casein Digest Agar Plate
(Triple Layer Pack, Gamma-Irradiated)
Soyabean Casein Digest Agar Slant
Soyabean Casein Digest Agar with 0.05% Polysorbate 80 and 0.07% Soya Lecithin Plate (Triple Layer Pack, Gamma-Irradiated)
Soyabean Casein Digest Agar with 0.5% Glycerol Plate (Triple Layer Pack, Gamma-Irradiated)
Soyabean Casein Digest Agar with 1% Glycerol Plate (Triple Layer Pack, Gamma-Irradiated)
Soyabean Casein Digest Agar with Soya Lecithin & 4% Polysorbate 20 Plate (Triple Layer Pack, Gamma-Irradiated)
Soyabean Casein Digest Agar
(Tryptone Soya Agar)
Soyabean Casein Digest Medium (Harmonized)
Soyabean Casein Digest Medium (Harmonized) III
Soyabean Casein Digest Medium (Sterility Testing Medium)
Soyabean Casein Digest Medium (Sterility Testing Medium) I
Soyabean Casein Digest Medium
(Tryptone Soya Broth)
Soyabean Casein Digest Medium VEG
Soyabean Casein Digest Medium
(Sterility Testing Medium) II
Soyabean Casein Digest Medium
(Sterility Testing Medium) III
Soyabean Casein Digest Medium
(Tryptone Soya Broth) (Harmonized)
Soyabean Casein Digest Medium
(Double Strength)
SS (Salmonella Shigella Agar) Agar
Standard Nutrient Agar
Starch Agar
Starch Casein Agar
Streptococcus Thermophilus Isolation Agar
Taurocholate Broth
TCBS Agar
TCBS Agar - Selective Media
Tellurite Glycine Agar Base
Tergitol-7 Agar Base
Test Kit for MR-VP
Tetrathionate Brilliant Green Bile Broth
Tetrathionate Brilliant Green Bile Broth IP
Tetrathionate Broth Base, Hajna
Toluidine Blue DNA Agar
Tributyrin Agar w/o Tributyrin
Triple Sugar Iron Agar
Triple Sugar Iron Agar BIS
Triple Sugar Iron Agar Slant
Tryptone Bile Agar
Tryptone Glucose Beef Extract Agar
(TGB Agar)
Tryptone Soya Agar with Lecithin and Polysorbate 80 (Soyabean Casein Digest Agar
with Lecithin and Polysorbate 80)
Tryptone Soya Yeast Extract Agar ISO
Violet Red Bile Agar BIS
Violet Red Bile Agar with MUG
Violet Red Bile Agar
(Crystal Violet Neutral Red Bile Lactose Agar)
Violet Red Bile Glucose Agar (Harmonized)
Violet Red Bile Glucose Agar IP
(Medium 6)
Violet Red Bile Glucose Agar Plate
(Harmonized)
Violet Red Bile Glucose Agar w/ Lactose
(Crystal Violet, Neutral Red, Bile Agar
with Glucose) (Agar Medium F) BP
Violet Red Bile Glucose Agar without Lactose ISO
Vogel Johnson Agar Base w/o Tellurite
Willis and Hobbs Medium Base
Xylose Lysine Deoxycholate Agar
Xylose Lysine Deoxycholate Agar (Medium 12) IP
Xylose Lysine Deoxycholate Agar Plate
(Harmonized)
Xylose Lysine Deoxycholate Agar, Modified ISO
Yeast Extract Chloramphenicol Dextrose Agar
Yeast Malt Agar (YM Agar)
YSG Agar
YSG Broth