MRS Agar (De Man, Rogosa and Sharpe Agar)

201131550500

A medium used for the cultivation of lactic acid bacteria from food.

Composition*

Ingredientsg/L
Enzymic Digest of Casein10.0
Cara Meat Extract#10.0
Yeast Extract4.0
Tri-Ammonium Citrate2.0
Sodium Acetate5.0
Magnesium Sulphate Heptahydrate0.2
Manganese Sulphate Tetrahydrate0.05
Di-potassium Hydrogen Phosphate2.0
Sorbitan Mono-oleate1.08
Glucose20.0
Agar12.4

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 5.7 ± 0.1
#Equivalent to Meat Extract

g/L: Suspend 66.73 g of the powder in 1000 mL purified / distilled water.

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