Ethyl Violet Azide Broth (E.V.A. Broth) BIS is a medium used for selective, confirmatory detection of Enterococci as an indicator of faecal pollution in water and other specimens in compliance with BIS specification IS:5887 (Part II):1976, reaffirmed 2005.
Composition*
| Ingredients | g/L |
| Casein Enzymic Hydrolysate | 20.0 |
| Dextrose | 5.0 |
| Dipotassium Phosphate | 2.7 |
| Monopotassium Phosphate | 2.7 |
| Sodium Chloride | 5.0 |
| Sodium Azide | 0.4 |
| Ethyl Violet | 0.00083 |
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.0 ± 0.2
g/L: Suspend 35.80 g of the powder in 1000 mL purified / distilled water.
