Burkholderia cepacia Selective Agar USP Plate is a medium used for the selective detection and isolation of Burkholderia cepacia complex from non-sterile and aqueous pharmaceutical and cosmetic products in compliance with USP.
Burkholderia cepacia Selective Agar is an opportunistic human pathogen that most often causes pneumonia in immunocompromised individuals with underlying lung disease (Such as cystic fibrosis or chronic granulomatous disease). B. cepacia is difficult to isolate on routinely used laboratory media like MacConkey Agar, since B. cepacia is a slow grower and therefore it is usually outgrown by the faster growing Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Burkhloderia Cepacia Selective Agar is based on PC medium, which was originally devised by Gilligan. This medium was found to be superior to MacConkey Agar for growth of B. cepacia. The medium is made selective for B. cepacia by the incorporation of bile salts, crystal violet and antibiotics. The antibiotics included are Polymyxin B, Gentamycin, Vancomycin in the form of freeze-dried supplement.
Composition*
| Ingredients | g/L |
| Casein Peptone | 10.0 |
| Lactose | 10.0 |
| Sucrose | 10.0 |
| Sodium Chloride | 5.0 |
| Yeast extract | 1.5 |
| Phenol red | 0.08 |
| Crystal violet | 2.0 mg |
| Agar | 14.0 |
| Gentamicin | 10.0 mg |
| Vancomycin | 2.5 mg |
| Polymyxin B | 600,000 U |
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 6.8 ± 0.3
