C.L.E.D. Agar with Andrade Indicator is used for isolation, enumeration and presumptive identification of microorganisms from urine, giving good colonial differentiation.
This electrolyte-deficient medium was modified for use in urine culture by substituting lactose and sucrose instead of mannitol and increasing the concentrations of bromothymol blue indicator and agar. The medium was further modified by the incorporation of cystine in order to enhance the growth of cystine dependent “dwarf colony” coliforms and by the deletion of sucrose. This new medium, Cystine-Lactose-Electrolyte-Deficient (C.L.E.D.) Agar is ideal for dip inoculation techniques and for urinary bacteriology in general.
Composition*
| Ingredients | g/L |
| Lactose | 10.0 |
| Tryptone | 4.0 |
| Peptone | 4.0 |
| Beef Extract | 3.0 |
| L-Cystine | 0.128 |
| Andrade Indicator | 0.10 |
| Bromothymol Blue | 0.02 |
| Agar | 15.0 |
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.5 ± 0.2
