Hartley Broth is used for cultivation of wide variety of bacteria from blood specially Streptococci and Corynebacterium diphtheriae.
Hartley described the value of tryptic digest of muscle for the production of diphtheria toxin. Since then it is used as a general-purpose broth, capable of initiating the growth of demanding (fastidious) organisms from a small inocula. Hartley's Digest Broth can be used for the recovery of fastidious organisms such as Group A, C, G Streptococci and Streptococcus pneumoniae from small inocula.
Composition*
Ingredients
| g/L
|
Tryptic Digest of Heart Muscle
| 29.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.6 ± 0.2
