Brain Heart Infusion with PABA and Agar

201020250500

MSDS
Technical Data

APPLICATION

Clinical Microbiology

Brain Heart Infusion (BHI) with para-aminobenzoic acid (PAB or PABA) is a medium used for the examination of blood from patients who have received sulfonamide therapy. Improved growth of anaerobes is seen on addition of agar.

Composition*

Ingredients 
g/L
Calf Brain, Infusion from 200 g 
7.7
Beef Heart, Infusion from 250 g 
9.8
Proteose Peptone 
10.0
Dextrose 
2.0
Sodium Chloride 
5.0
Disodium Phosphate 
2.5
p-Aminobenzoic Acid 
0.050
Agar 
1.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 7.4 ± 0.2

g/L: Suspend 38.05 g of the powder in 1000 mL purified / distilled water and mix thoroughly.

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