Fraser Secondary Enrichment Broth Base

201060140500

Fraser Secondary Enrichment Broth Base is used for isolation, cultivation and enrichment of Listeria monocytogenes from food and environmental samples.

Composition* 

Ingredients 
g/L
Proteose Peptone
5.0
Casein Enzymic Hydrolysate
5.0
Yeast Extract
5.0
Cara Beef Extract#
5.0
Sodium Chloride
20.0
Disodium Phosphate
12.0
Lithium Chloride 
3.0
Monopotassium Phosphate
1.35
Esculin 
1.0
Ferric Ammonium Citrate
0.5

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 7.2 ± 0.2
#Equivalent to Beef Extract

g/L: Suspend 57.85 g of the powder in 990 mL purified / distilled water and mix thoroughly.

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