Thioglycollate Broth is a medium used for cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria and for aseptic process simulations.
Thioglycollate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycollate in the medium consumes oxygen and permits the growth of obligate anaerobes. This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth. The oxygen concentration at a given level is indicated by a redox-sensitive dye such as resazurin that turns pink in the presence of oxygen. This allows the differentiation of obligate aerobes, obligate anaerobes, facultative anaerobes, microaerophiles and aerotolerant organisms. For example, obligately anaerobic Clostridium species will be seen growing only in the bottom of the test tube.
Composition*
| Ingredients | g/L |
| Tryptone | 15.0 |
| Dextrose | 5.5 |
| Yeast Extract | 5.0 |
| Sodium Chloride | 2.5 |
| Sodium Thioglycollate | 0.5 |
| L-Cystine | 0.5 |
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.1 ± 0.2
