MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Taurocholate is used for the cultivation and differentiation of enteric bacteria and potentially pathogenic Gram-positive organisms while restricting swarming of Proteus species.
MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens. Subsequently MacConkey Agar and Broth have been recommended for use in microbiological examination of foodstuffs and for direct plating / inoculation of water samples for coliform counts.
Composition*
Ingredients
| g/L
|
Peptone
| 20.0
|
Lactose
| 10.0
|
Neutral Red
| 0.04
|
Sodium Taurocholate
| 5.0
|
Agar
| 20.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
