Letheen Broth is a medium recommended for determination of bactericidal activity of quaternary ammonium compounds using Escherichia coli or Staphylococcus aureus.
Letheen Broth is used to determine the phenol co-efficient of cationic surfactants as recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC). It is also used in hygiene swabbing protocols where it is necessary to neutralize quaternary ammonium compounds. A modification of FDA Broth, Letheen Broth is easily prepared and has a clear appearance aiding in visual inspection for growth.
Composition*
| Ingredients | g/L |
Peptic Digest of Animal Tissue
| 10.0
|
Beef Extract
| 5.0
|
Sodium Chloride
| 5.0
|
Lecithin
| 0.7
|
Polysorbate 80
| 5.0ml
|
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.0 ± 0.2
