Columbia Agar is used for detection of Clostridium sporogenes from pharmaceutical products in accordance with the microbial limit testing by harmonized methodology of USP/EP/BP/JP/IP.
Columbia Blood Agar Base used as a general-purpose nutritious medium was devised by Ellner et al., from Columbia University, which was further enriched by the addition of sheep blood. It can also be used for the isolation of organisms by addition of various supplements. Columbia Agar is prepared in accordance with the microbial limit testing harmonized methodology of USP/EP/BP/JP/IP. This medium is recommended to check the presence of Clostridium in non-sterile products like food, dietary, nutritional supplements related products.
Composition*
| Ingredients | g/L |
| Pancreatic Digest of Casein | 10.0 |
| Meat Peptic Digest | 5.0 |
| Heart Pancreatic Digest | 3.0 |
| Yeast Extract | 5.0 |
| Maize Starch | 1.0 |
| Sodium Chloride | 5.0 |
| Agar | 15.0 |
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.3 ± 0.2
