BHI Broth is a highly nutritious general-purpose liquid medium for cultivation of variety of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria.
BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. This medium is especially useful as a growth and suspension medium for Staphylococci which is to be tested for coagulase production and when supplemented with yeast extract, hemin and menadione, it was found to be better in producing heavy growth of five species of BHI agar is included in the Bacteroides. Bacteriological Analytical Manual for food and cosmetics testing and is also recommended by APHA for the examination of foods and milk.
Composition*
| Ingredients | g/L |
| Beef Heart Infusion from 250 g | 9.8 |
| Calf Brain Infusion from 200 g | 7.7 |
| Proteose Peptone | 10.0 |
| Sodium Chloride | 5.0 |
| Dextrose | 2.0 |
| Disodium Phosphate | 2.5 |
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
