Hugh Leifson Medium

201080030500

Hugh Leifson Medium is used to distinguish between anaerobic and aerobic breakdown of carbohydrate (glucose).

Composition*

Ingredients 
g/L
Peptone  
2.0
Dipotassium Phosphate
0.3
Sodium Chloride 
5.0
Glucose (Dextrose)
10.0
Bromothymol Blue 
0.05
Agar 
2.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C):  6.8 ± 0.2

g/L: Suspend 19.35 g of the powder in 1000 mL purified / distilled water. 

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