Triple Sugar Iron Agar is used for the identification of Gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production.
Composition*
Ingredients
| g/L
|
Lactose
| 10.0
|
Sucrose
| 10.0
|
Peptone
| 10.0
|
Tryptone
| 10.0
|
Sodium Chloride
| 5.0
|
Yeast Extract
| 3.0
|
Cara Beef Extract#
| 3.0
|
Dextrose
| 1.0
|
Sodium Thiosulphate
| 0.3
|
Ferrous Sulphate
| 0.2
|
Phenol Red
| 0.024
|
Agar
| 12.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
#Equivalent to Beef Extract
g/L: Suspend 64.52 g of powder in 1000 mL purified / distilled water.
