Brain Heart Infusion (BHI) with para-aminobenzoic acid (PAB or PABA) is a medium used for the examination of blood from patients who have received sulfonamide therapy. Improved growth of anaerobes is seen on addition of agar.
Composition*
Ingredients
| g/L
|
MX Nutrients 2#
| 7.7
|
MX Nutrients 1##
| 9.8
|
Proteose Peptone
| 10.0
|
Dextrose
| 2.0
|
Sodium Chloride
| 5.0
|
Disodium Phosphate
| 2.5
|
p-Aminobenzoic Acid
| 0.050
|
Agar
| 1.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.4 ± 0.2
#Equivalent to intended performance of Calf Brain, Infusion from 200 g
##Equivalent to intended performance of Beef Heart, Infusion from 250 g
g/L: Suspend 38.05 g of the powder in 1000 mL purified / distilled water and mix thoroughly.
