TCBS Agar - Selective Media

201200020500

This medium is recommended for the selective isolation of Vibrio cholerae and other enteropathogenic Vibrios.

Composition* 

Ingredients
g/L
Peptone, Special
10.0
Yeast Extract
5.0
Sodium Citrate
10.0
Sodium Thiosulphate
10.0
Sodium Cholate
3.0
Gall Powder#
5.0
Sucrose
20.0
Sodium Chloride
10.0
Ferric Citrate
1.0
Bromothymol Blue
0.04
Thymol Blue
0.04
Agar
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 8.8 ± 0.2
#Equivalent to intended performance of Oxgall

g/L: Suspend 89.08 g of the powder in 1000 mL purified / distilled water.

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