Kligler Iron Agar ISO

201110080100 / 201110080500

Kligler Iron Agar is a differential medium recommended for identification of Pseudomonas species and members of Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to produce hydrogen sulphide in compliance with ISO specification ISO/13720:1995.

Composition*

Ingredients 
g/L
Casein Enzymic Hydrolysate
20.0
Beef Extract
3.0
Yeast Extract
3.0
Sodium Chloride
5.0
Lactose
10.0
Glucose Anhydrous
1.0
Ferrous Ammonium Sulphate. 6H2O
0.50
Sodium Thiosulphate Pentahydrate
0.50
Phenol Red
0.025
Agar
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 7.4 ± 0.2

g/L:  Suspend 57.70 g of the powder in 1000 mL purified / distilled water.

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