B12 Maintenance medium is used for the propagation, cultivation and maintenance of E. coli mutant 113-3D, ATCC 11105, which is the test organism in vitamin B12 assay.
Composition*Ingredients
| g/L
|
Sucrose
| 12.0
|
Yeast Extract
| 5.0
|
Tryptone
| 5.0
|
Magnesium Sulphate
| 0.2 |
Monopotassium Phosphate
| 0.5
|
Sodium Chloride
| 0.1 |
MX Nutrients 3#
| 0.05 |
Ferrous Sulphate
| 0.001
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C): 7.0 ± 0.2
#Equivalent to intended performance of Liver Extract
g/L: Suspend 37.85 g of the powder in 1000 mL purified / distilled water.
