B12 Assay Medium (Lactobacillus leichmannii Culture) is used for determining Vitamin B12 concentration by the microbiological assay technique.
composition*
| Ingredients | g/L |
| Vitamin Assay Casamino Acids | 15.0 |
| Dextrose | 40.0 |
| Asparagine | 0.2 |
| Sodium Acetate | 20.0 |
| Ascorbic Acid | 4.0 |
| L-Cystine | 0.4 |
| DL-Tryptophan | 0.4 |
| Adenine Sulphate | 0.02 |
| Guanine Hydrochloride | 0.02 |
| Uracil | 0.02 |
| Xanthine | 0.02 |
| Riboflavin | 0.001 |
| Thiamine Hydrochloride | 0.001 |
| Biotin | 0.00001 |
| Niacin | 0.002 |
| p-Aminobenzoic Acid | 0.002 |
| Calcium Pantothenate | 0.001 |
| Pyridoxine Hydrochloride | 0.004 |
| Pyridoxal Hydrochloride | 0.004 |
| Pyridoxamine Hydrochloride | 0.0008 |
| Folic Acid | 0.0002 |
| Monopotassium Phosphate | 1.0 |
| Dipotassium Phosphate | 1.0 |
| Magnesium Sulphate | 0.4 |
| Sodium Chloride | 0.02 |
| Ferrous Sulphate | 0.02 |
| Manganese Sulphate | 0.02 |
| Polysorbate 80 | 2.0 |
*Adjusted to suit performance parameters
Final pH (at 25°C) 6.0 ± 0.1
g/L: Suspend 85.00 g of the powder in 1000 mL of purified / distilled water.
