Ready to use combi kit each of Albert's Stain A and Albert's Stain B for observing metachromatic granules.
Albert's Metachromatic Stain demonstrates the presence of metachromatic granules found in
Corynebacterium diphtheriae. The granules stain purple-black against the light green counterstained cytoplasm.
This helps to distinguish Corynebacterium diphtheriae from most of the short non-pathogenic diphtheroides
which lack granules.
Reagent / Contents
Albert’s Stain A:
Toluidine blue
| 0.15 g
|
Malachite green
| 0.20 g
|
Glacial acetic acid
| 1.0 mL
|
Ethyl alcohol, 95%
| 2.0 mL
|
Distilled water
| 100 mL
|
Appearance: Peacock blue coloured liquid.
Albert’s Stain B:
Iodine crystals
| 2.0 g
|
Potassium iodide
| 3.0 g
|
Distilled water
| 300 mL
|
Appearance: Dark amber coloured solution.
