Leishman's Stain is used in microscopy for staining of blood smears.
Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally
used to differentiate and identify leucocytes, malaria parasites, and Trypanosoma. It is based on a methanolic
mixture of "polychromed" methylene blue. (i.e., demethylated into various azures) and eosin. The methanolic stock
solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step.
Reagents / Contents:
Leishman stain
| 0.15 g
|
Methanol, absolute
| 100 mL
|
**Formula adjusted, standardized to suit performance parameters
Appearance: Blue colour solution.
