Field's stain A is a histological method for staining of blood smears.
Field's stain is a version of a Romanowsky stain, used for rapid processing of the specimens. Polychromated methylene blue and eosin stains selectively basophilic and acidophilic cellular elements to demonstrate blood cells and hemo-parasites. This staining method is used for screening thick films of malarial parasites.
Reagents / Contents :
Methylene blue
| 1.45 g
|
Disodium hydrogen phosphate
| 9.09 g
|
Azur II
| 0.91 g
|
Potassium dihydrogen orthophosphate
| 11.36 g
|
Distilled water
| 977.2 mL
|
**Formula adjusted, standardized to suit performance parameters
Appearance:
Field's Stain A is a dark blue coloured solution.