Ready to use combi kit each of Leishman Stain and Phosphate Buffer (pH 7) used in microscopy for staining blood smears.
Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally
used to differentiate and identify leucocytes, malaria parasites, and Trypanosoma. It is based on a methanolic
mixture of "polychromed" methylene blue. (i.e., demethylated into various azures) and eosin. The methanolic stock
solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. The buffer
system consists of two salts: sodium hydrogen phosphate (Na₂HPO₄) and potassium dihydrogen phosphate
(KH₂PO₄). Due to their concentration and pH, they provide a stable environment for staining.
Reagent / Contents:Leishman Stain
|
|
Leishman stain
| 0.15 g
|
Methanol, absolute
| 100 mL
|
Phosphate Buffer (pH7)
|
|
KH2PO4
| 8 mM
|
Na2HPO4
| 6 mM
|
Final pH (at 25°C)
| 7.0
|
**Formula adjusted, standardized to suit performance parameters
Appearance:
Leishman Stain: Blue colour solution
Phosphate Buffer (pH7): Clear colourless solution