Leishman Stain Kit

207120670250 / 207120670500

Ready to use combi kit each of Leishman Stain and Phosphate Buffer (pH 7) used in microscopy for staining blood smears.

Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally used to differentiate and identify leucocytes, malaria parasites, and Trypanosoma. It is based on a methanolic mixture of "polychromed" methylene blue. (i.e., demethylated into various azures) and eosin. The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. The buffer system consists of two salts: sodium hydrogen phosphate (Na₂HPO₄) and potassium dihydrogen phosphate (KH₂PO₄). Due to their concentration and pH, they provide a stable environment for staining.


Reagent / Contents:

Leishman Stain

Leishman stain
0.15 g 
Methanol, absolute
100 mL
Phosphate Buffer (pH7) 

KH2PO4
8 mM
Na2HPO4
6 mM 
Final pH (at 25°C) 
7.0

**Formula adjusted, standardized to suit performance parameters


Appearance:

Leishman Stain: Blue colour solution

Phosphate Buffer (pH7): Clear colourless solution



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