Ready to use combi kit each of Giemsa Stain and Phosphate Buffer (pH 6.8) for cytogenetics and histopathological diagnosis.
Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA
and attaches itself to where there are high amounts of adenine-thymine bonding. Giemsa stain is mainly used for
staining of peripheral blood smears and specimens obtained from the bone marrow. It is used to obtain differential
white blood cell counts. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify
chromosomal aberrations. It is commonly used for G-Banding (Giemsa-Banding). The buffer system consists of
two salts: sodium hydrogen phosphate (Na₂HPO₄) and potassium dihydrogen phosphate (KH₂PO₄). Due to their
concentration and pH, they provide a stable environment for staining.
Reagents / contents:
| Giemsa Stain | |
| Azur II eosin | 3.0 g |
| Azur II | 0.8 g |
| Glycerine | 125 mL |
| Methyl alcohol, absolute | 375 mL |
| Phosphate Buffer (pH 6.8) | |
| KH2PO4 | 8 mM |
| Na2HPO4 | 6 mM |
| Final pH (at 25°C) | 6.8 |
**Formula adjusted, standardized to suit performance parameters
Appearance:
Giemsa Stain: Dark blue coloured solution.
Phosphate Buffer (pH 6.8): Clear colourless solution
