H & E Staining method is used for the routine staining of the cationic and anionic tissue components in tissue sections. This is the standard reference stain used in the study of histochemical tissue pathology.
Hematoxylin and eosin stain (abbreviated as H & E stain) is one of the principal tissue stains used in routine histology staining methods. It is the most widely used stain in medical diagnosis and is often the gold standard; wherein, when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H & E. H & E Stain is the combination of two histological stains: Hematoxylin and Eosin. The hematoxylin is a selective nuclear stain which stains the cell nuclei blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors. The stain shows the general layout and distribution of cells and provides a general overview of a tissue sample's structure. Hence, there is a clear differentiation between the nuclear and cytoplasmic parts of a cell.
Reagents / Contents:
1. Hematoxylin Harris
| Hematoxylin | 5.0 g |
| Ammonium/ Potassium Alum | 100 g |
| Mercuric Oxide | 2.5 g |
| Alcohol 95% | 50 mL |
| Distilled Water | 1000 mL |
Appearance: Maroon purplish solution.
2. Eosin (AQU.) 2%
| Eosin-Y | 2.0 g |
| Distilled water | 100 mL |
Appearance: Dark reddish solution.
