B12 Maintenance Medium
(for E. coli mutant)

201020030100

MSDS
Technical Data

APPLICATION

Pharmaceuticals

B12 Maintenance medium is used for the propagation, cultivation and maintenance of E. coli mutant 113-3D, ATCC 11105, which is the test organism in vitamin B12 assay.

Composition*

Ingredients 
g/L
Sucrose 
12.0
Yeast Extract
5.0
Tryptone
5.0
Magnesium Sulphate 
0.2
Monopotassium Phosphate
0.5
Sodium Chloride
0.1
Liver Extract
0.05
Ferrous Sulphate
0.001
Agar
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25°C): 7.0 ± 0.2

g/L: Suspend 37.85 g of the powder in 1000 mL purified / distilled water.

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