B12 Assay Agar
(using E. coli Mutant Culture)

201020010100

MSDS
Technical Data

APPLICATION

Pharmaceuticals

B12 Assay Agar (using E. coli mutant culture) is recommended for the microbiological assay of Vitamin B12 by the cup plate or disc plate method.

Composition*

Ingredients
g/L
Dipotassium Phosphate
14.0
Monopotassium Phosphate 
6.0
Ammonium Chloride 
5.0
Glucose 
5.0
DL- Asparagine 
3.0
Ammonium Nitrate 
2.0
Sodium Chloride 
1.0
L- Arginine Hydrochloride  
0.2
Magnesium Sulphate 
0.2
Ammonium Sulphate 
0.1
Calcium Chloride 
0.001
Zinc Sulphate
 0.00009
Ammonium Molybdate 
 0.00001
Borax 
0.00001
Ferrous Sulphate
 0.000054
Manganese Chloride
 0.000046
Copper Sulphate 
0.000025
Agar 
15.0

*Adjusted to suit performance parameters.
  Final pH (at 25˚C): 7.2 ± 0.2

  g/L: Suspend 51.50 g of the powder in 1000 mL purified / distilled water.

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