Amies with Charcoal Transport Medium is recommended for transportation and preservation of microbiological specimen.
The transport medium should be semi-solid, non-nutritive, and reductive and should be able to hamper self-destructive enzymatic reactions within the cells and in addition, must inhibit toxic oxidation reactions. Amies modified Stuart’s Transport Medium by replacing glycerophosphate with an inorganic phosphate buffer and adding charcoal to the medium. This modified medium gave a higher percentage of positive results than the transport medium of Stuart.
Composition*
Ingredients
| g/L
|
Sodium Chloride
| 3.0
|
Potassium Chloride
| 0.2
|
Calcium Chloride
| 0.1
|
Magnesium Chloride
| 0.1
|
Monopotassium Phosphate
| 0.2
|
Disodium Phosphate
| 1.15
|
Sodium Thioglycollate
| 1.0
|
Charcoal
| 10.0
|
Agar
| 4.0
|
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.3 ± 0.2
