Amies Transport Medium without Charcoal and with Cotton Swab is recommended for transportation and preservation of microbiological specimen.
Transport medium is necessarily and should be non-nutrient, semisolid, reductive medium which hampers the self destructive enzymatic reactions within the cells and also inhibits toxic oxidation effects. Amies Transport Medium without Charcoal was formulated by Amies by modifying Cary and Blair’s medium for transporting microbiological specimens. Amies transport medium is a modification of Stuarts Transport Medium, in which glycerophosphate is replaced by inorganic phosphate buffer. This prevents problem of overgrowth of contaminating organisms while carrying faecal specimens containing Shigellae which derive energy from glycerophosphate.
Composition*
| Ingredients | g/L |
| Sodium Chloride | 3.0 |
| Potassium Chloride | 0.2 |
| Calcium Chloride | 0.1 |
| Magnesium Chloride | 0.1 |
| Monopotassium Phosphate | 0.2 |
| Disodium Phosphate | 1.15 |
| Sodium Thioglycollate | 1.0 |
| Agar | 4.0 |
*Adjusted to suit performance parameters.
Final pH (at 25°C) 7.3 ± 0.2
