EMB Agar, Levine Plate is used for isolation, enumeration and differentiation of members of Enterobacteriaceae.
This medium is recommended for the isolation, enumeration and differentiation of members of the coliform group by American Public Health Association. This formulation gives a sharp and distinct differentiation between the colonies of lactose fermenting and nonlactose fermenting microorganisms.
Composition*
Ingredients
| g/L
|
Pancreatic Digest of Gelatin
| 10.0
|
Lactose
| 10.0
|
Dibasic Potassium Phosphate
| 2.0
|
Eosin Y
| 0.4
|
Methylene Blue
| 0.065
|
Agar
| 15.0
|
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.1 ± 0.2
