Gram Safranin 0.5%

207070200125 / 207070200250 / 207070200500

Gram Safranin 0.5% is used as a counterstain in Gram Staining.

Various mechanisms have been proposed to explain the Gram character. The characteristic colour of Gram positive bacteria results from the ability of a cell to resist the loss of the primary stain during the decolourization step, as compared to the gram-negative cells which do not possess this ability. Most researchers who have studied the mechanism of Gram stain reaction have concluded that the mechanism is based on the distinctive chemistry and physical structure of the cell walls of the organisms.

Reagents/Contents:

Safranin O
0.5 gm 
Ethyl alcohol
10.0 mL
Distilled water 
100.0 mL


Appearance: Red coloured solution



ENQUIRE NOW