Willis and Hobbs Supplement

An antibiotic supplement recommended for isolation of Clostridium perfringens and Clostridium botulinum species.

Willis and Hobbs Supplement is used with Willis and Hobbs Medium Base for selective isolation of Clostridium perfringens and Clostridium botulinum species from food. This medium formulated by Willis and Hobbs can be used for the identification of Clostridium species on the basis of lecithinase reaction in egg yolk and lactose fermentation. This medium is prepared in accordance with Indian Standard under the specifications IS: 5887 (Part-IV) 1976.

Each vial, sufficient for 475 mL medium contains: