C.L.E.D. Agar with Bromothymol Blue Plate is used for isolation and differentiation of urinary tract pathogens on the basis of lactose fermentation.
Sandy’s observed that restricting the electrolytes on a solid’ medium might prevent the swarming of Proteus. This electrolyte medium was modified for use in urine culture by substituting lactose and sucrose instead of mannitol and increasing the concentrations of bromothymol blue indicator and agar. The medium was further modified by the incorporation of cystine in order to enhance the growth of cystine-dependent “dwarf colony” coliforms and by the deletion of sucrose.
Composition*
| Ingredients | g/L |
| Pancreatic Digest of Gelatin | 4.0 |
| Pancreatic Digest of Casein | 4.0 |
| Beef Extract | 3.0 |
| Lactose | 10.0 |
| L-cystine | 128.0 |
| Bromothymol Blue | 0.02 |
| Bacteriological Agar | 15.0 |
*Adjusted to suit performance parameters.
pH at 25°C ± 2°C: 7.3 ± 0.2
