Giemsa stain is used for cytogenetics and for the histopathological diagnosis.
Giemsa stain is a mixture of methylene blue, eosin, and azure B. It is specific for the phosphate groups of DNA
and attaches itself to where there are high amounts of adenine-thymine bonding. Giemsa stain is mainly used for
staining of peripheral blood smears and specimens obtained from the bone marrow. It is used to obtain differential
white blood cell counts. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify
chromosomal aberrations. It is commonly used for G-Banding (Giemsa-Banding).
Reagents/ contents:
Azur II eosin
| 3.0 g
|
Azur II
| 0.8 g
|
Glycerine
| 125 mL
|
Methyl alcohol, absolute
| 375 mL
|
**Formula adjusted, standardized to suit performance parameters.
Appearance: Dark blue coloured solution.
