Gram Crystal Violet is used as staining solution for monochrome staining of microbes.
Various mechanisms have been proposed to explain the Gram character. The characteristic colour of Gram positive bacteria results from the ability of a cell to resist the loss of the primary stain during the decolorization step,
as compared to the gram-negative cells which do not possess this ability. Most researchers who have studied the
mechanism of Gram stain reaction have concluded that the mechanism is based on the distinctive chemistry and physical structure of the cell walls of the organisms.
Reagents/Contents:
Crystal violet
| 2.0 gm
|
Ethyl alcohol
| 20.0 ml
|
Ammonium oxalate, monohydrate
| 0.8 gm
|
Distilled water
| 80.0 m
|
**Formula adjusted, standardized to suit performance parameters
Appearance: Violet coloured solution.
