Combicult® is a ready to use, combipack of solid and liquid medium for Mycobacterium tuberculosis isolation.
The gold standard for primary isolation of Mycobacterium tuberculosis is the use of liquid media in conjunction with solid media. Most Mycobacterium species grow more quickly in liquid media than solid media. Liquid media also support higher detection rates especially with specimen material containing smaller number of bacilli. Simultaneous inoculation of solid media and liquid media yields significantly higher recovery rates for Mycobacterium tuberculosis growth as compared to when each media is used independently.
Reagent:
Lowenstein Jensen medium is provided as a ready to use slant. Kirchner medium is provided as a three-component medium:
a) Kirchner medium base.
b)Kirchner selective enrichment containing antibiotic cocktail Polymyxin B, Amphotericin B, Carbenicillin, Trimethoprim (PACT)
c) Sterile distilled water for reconstitution of Kirchner selective enrichment.