For detection of Esculin Hydrolysis in the presence of bile.
Directions :
1. Prepare Mueller Hinton Agar Plates.
2. Inoculate the entire agar surface of the plate three times, rotating the plate 60° between streaking to obtain even inoculation.
3. Swab the rim of the agar bed too.
4. Immediately place Bile esculin disc in the centre of the plate.
5. Tap the discs with some sterile needle or forceps after placing them on the agar for complete contact with the medium surface.
6. Invert the plates and incubate at 35°C-37°C for 18-24 hours.
Appearance: Blank Filter paper discs of 6mm diameter.
