PYR Reagent is used for detection of pyroglutamate aminopeptidase activity in group A Streptococci and Enterococci.
PYR is a rapid test to determine the ability of the organism to produce the enzyme L-pyrroglutamyl-aminopeptidase. Following incubation with a PYR substrate and addition of the PYR Reagent (cinnamaldehyde), a cherry red colour development indicates a positive test. The colour is formed when the PYR reagent combines with L-pyrrolidone and b-naphthylamine, hydrolysis products from the substrate, L-pyrrolidonyl-b-napthylamide breakdown.
Reagents/contents :
The PYR Reagent comprises of:
1. 1% Cinnamaldehyde.
Appearance: Amber coloured clear solution.
