The reagent is used in Voges-Proskauer test for detection of acetoin production by bacterial culture.
The VP test is used to identify microbes that ferment glucose and produce neutral, non-acidic end products. After glycolysis (glucose fermentation), these organisms convert pyruvic acid formed into acetyl methyl carbinol (acetoin) or its reduction product 2,3-butanediol. Since 2,3-butanediol cannot be detected directly, this test indirectly determines its presence by testing for acetoin. Acetoin is oxidized to diacetyl in the presence of Baritt Reagent A (α- Naphthol) and Baritt Reagent B (Potassium Hydroxide). Diacetyl, in turn reacts with Creatine, which acts as a catalyst to produce a red coloured complex.
Reagents/contents :
The Microxpress® Baritt A and B reagents comprise of:
1. Baritt Reagent A: 5% α- Naphthol in absolute alcohol.
2. Baritt Reagent B: 40% Potassium Hydroxide.
Appearance: Colourless clear liquid.
